Fluorescence microscopy has become a popular mainstay of subcellular visualization, but its resolving power is limited by the diffraction of light. Several approaches have been taken to break the ...
Nanoscopy is a field of microscopy that focuses on imaging and studying structures and processes at the nanoscale, typically below the diffraction limit of light. It encompasses various techniques ...
The Abbe diffraction barrier, which fundamentally restricted the lateral optical resolution to 200 – 300 nm until the early 1990s, can be overcome by super-resolution optical fluorescence imaging ...
Stimulated emission depletion (STED) microscopy has shown that diffraction-unlimited spatial resolution is viable with conventional lenses and visible light. Stefan W Hell, Lars Kastrup and Katrin I ...
Schematic of the planar device for producing dark- field speckle patterns. (Image from USTC). Credit: Douguo Zhang et al. Researchers from the University of Science and Technology of China (USTC) have ...
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